Thermo Fisher Scientific ASH2L Polyclonal Antibody
Thermo Fisher Scientific의 ASH2L Polyclonal Antibody는 인간, 마우스, 랫트에서 반응하며 Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제된 비결합형 액상 항체로, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ASH2L. Recombinant protein control fragment (Product #RP-102513). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638290 |
Product Specific Information
Immunogen sequence:
TTGTTKKARS DPLFSAQRLP PHGYPLEHPF NKDGYRYILA EPDPHAPDPE KLELDCWAGK PIPGDLYRAC LYERVLLALH DRAPQLKISD DRLTVVGEKG YSMVRASHGV RKGAWYFEIT VDEM
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1 (isoform 1), which contains a nuclear localization signal and PHD finger motif, suggesting transcription regulatory function.
Predicted molecular weight: 68.7 kDa
Alternative splicing may result in isoforms at 60 kDa and 56.5 kDa.
Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates gene expression in early development. Homology to mouse ASH2L is 90%.
Expression profile:
- Predominantly in adult heart and testis, fetal lung and liver
- Barely detectable in adult lung, liver, kidney, prostate, and peripheral leukocytes
- Expressed in leukemia cell lines (K562, Hel, Dami) with erythroid and megakaryocytic potential
- Expression varies under differentiation inducers (e.g., phorbol ester, hemin), suggesting a role in hematopoiesis and leukemia association.
ASH2L has been suggested to interact with beta-tubulin and beta-catenin.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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