Thermo Fisher Scientific RNASE3 Monoclonal Antibody (5G4)
Human RNASE3 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot, IHC, ICC, Flow cytometry에 사용 가능. 단백질 G 정제, 2 mg/mL 농도, PBS/glycerol/BSA buffer에 저장. 연구용으로만 사용.
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Thermo Fisher Scientific RNASE3 Monoclonal Antibody (5G4)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5G4 |
| Immunogen | Recombinant protein within Human Ribonuclease 3 aa 8–140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848837 |
Product Specific Information
Positive Control: U937, HeLa, SHG-44, human spleen tissue, human kidney tissue.
Target Information
ECP (eosinophil cationic protein), also known as RNS3 or RNASE3 (ribonuclease, RNase A family, 3), is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family.
ECP localizes to the granule matrix of eosinophils and is involved in the immune response system. It acts as a cytotoxin, neurotoxin, and helminthotoxin secreted by activated human eosinophils.
ECP exhibits anti-parasitic, antibacterial, and ribonucleolytic activities. It has been associated with the neurotoxic Gordon phenomenon, characterized by ataxia, muscular rigidity, paralysis, and tremor.
ECP is a marker of eosinophilic inflammation and is elevated in active asthma and other allergic diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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