Thermo Fisher Scientific p150glued Polyclonal Antibody
Rabbit polyclonal antibody against human p150glued (Dynactin 1). Validated for WB and IP. Antigen affinity purified, supplied in liquid form at 1 mg/mL. Suitable for research use only, stored at 4°C.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1100–1150 of human 150 kDa Dynein-Associated Polypeptide (Dynactin 1, UniProt ID: Q14203-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from the date of receipt.
Target Information
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in various cellular functions, including:
- ER-to-Golgi transport
- Centripetal movement of lysosomes and endosomes
- Spindle formation and chromosome movement
- Nuclear positioning and axonogenesis
This subunit interacts with the dynein intermediate chain and binds microtubules through a conserved CAP-Gly domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene are associated with distal hereditary motor neuronopathy type VIIB (HMN7B), also known as distal spinal and bulbar muscular atrophy (dSBMA).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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