Thermo Fisher Scientific ETS1 Recombinant Rabbit Monoclonal Antibody (ARC0082)
Recombinant rabbit monoclonal antibody specific for human ETS1 protein. Validated for Western blot and ELISA applications. Produced in HEK293 cells and purified by affinity chromatography. Supplied as liquid in PBS with glycerol and BSA, for research u...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0082 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human ETS1 (P14921) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849218 |
Product Specific Information
Immunogen sequence:
MKAAVDLKPT LTIIKTEKVD LELFPSPDME CADVPLLTPS SKEMMSQALK ATFSGFTKEQ QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein and a member of the DNA-binding ETS protein family. It is a multifunctional protein with an N-terminal pointed domain, a central transactivation domain, and two auto-inhibitory domains flanking the conserved C-terminal ETS domain.
ETS1 interacts with factors such as PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase. Activated by Ras/MAPK signaling, its expression is upregulated in resting T-cells. Activity is modulated by AP-1, Sp-1, c-myb, and MafB.
Sp100 activates ETS1 in nuclear bodies, while interaction with EAP1/Daxx represses MMP1 and BCL2 transactivation. ETS1 also interacts with STAT6 to modulate Socs-1 expression induced by IL-4 and with GATA3 to regulate Tax-1–dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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