Thermo Fisher Scientific I-Ab/I-Ad Monoclonal Antibody (25-9-17S), FITC
Mouse I-Ab/I-Ad 단백질을 인식하는 FITC 결합 단클론 항체로 Flow Cytometry에 적합. MHC Class II 관련 면역 연구용. 단클론 IgG2a 형이며 Protein G로 정제됨. 4°C 단기, -20°C 장기 보관 권장.
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Thermo Fisher Scientific I-Ab/I-Ad Monoclonal Antibody (25-9-17S), FITC
Applications
- Flow Cytometry (FACS)
Tested Dilution: 0.5 µg/1x10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25-9-17S |
| Immunogen | C3H.SW splenocytes |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4–5 mg/mL BSA |
| Contains | 0.02% Sodium Azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539160 |
Additional Formats
- Biotin conjugate: MA5-17775
- PE conjugate: MA5-17777
- Custom conjugation available upon request
Product Specific Information
MA5-17776 targets I-Ab/I-Ad in FACS applications and shows reactivity with mouse samples.
The immunogen used is C3H.SW splenocytes.
Target Information
MHC class II molecules are heterodimers composed of non-covalently associated alpha (31–34 kDa) and beta (26–29 kDa) chains.
Antigen presentation via MHC class II requires lysosomal proteases for processing endocytosed antigens into peptides and for degradation of the invariant chain (Ii), enabling peptide binding.
These molecules present exogenous antigens to CD4+ T lymphocytes (T helper cells). CD4 interacts with non-polymorphic residues of MHC class II.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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