Takara Apoptosis assay: In Situ Apoptosis Detection Kit

Overview

• Quick and easy detection of apoptotic cells
• Sensitive detection of single cells in the initial stages of apoptosis
• Specific staining of apoptotic but not necrotic cells
• Flexible enough to be used with tissue section or fixed-cell samples
• Individual components are available separately
• Control slide supplied for accuracy
• Non-radioactive

More Information

Applications

• Fragmented-DNA detection by the TUNEL method
• Detection of DNA strand breaks in apoptotic cells via fluorescence or light microscopy

Fragmented DNA detection by the TUNEL method

• Type: direct TUNEL labeling assay

• Used for: detection of DNA strand breaks in apoptotic cells by flow cytometry or fluorescence microscopy

• Used to assay: cells in suspension (permanent cell lines, normal and tumor cells ex vivo), adherent cells, cytospins, cell smears, frozen or paraffin-embedded tissue sections

• Technique: end-labeling of DNA with fluorescein-dUTP, followed by direct analysis of fluorescent cells

• Time required: 1–2 hr (Not including sample preparation, permeabilization, etc.)

• Detection method: fluorescence and light microscopy

• Number of Assays per Kit: 20

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.