Thermo Scientific™

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

This substrate bundle contains SuperSignal West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate자세히 알아보기

This substrate bundle contains SuperSignal West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

SuperSignal West Femto Maximum Sensitivity Substrate
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is a high-sensitivity enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult-to-detect targets.

• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to eight hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

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SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate
Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

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사양

제품 유형Substrate

검출 방법Chemiluminescence

형태Liquid

용도(애플리케이션)Western Blot

Membrane CompatibilityNitrocellulose, PVDF

제품라인SuperSignal™

수량100 mL + 20 mL bundle

SubstrateHorseradish Peroxidase

민감도Low-femtogram (mid-zeptomole)

충분12 Mini-Gel Blots, 1000 sq. cm of Membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane

배송 조건Approved for shipment at Room Temperature or on Wet or Dry Ice

Unit SizeEach