Thermo Scientific™

SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

This substrate trial pack contains sample sizes of two substrates: SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal West자세히 알아보기

This substrate trial pack contains sample sizes of two substrates: SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate
Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

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SuperSignal West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for routine western blotting applications.

• Sensitivity: picogram to femtogram
• Stability: eight hours for working solution; one year for kit at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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사양

제품 유형Substrate

검출 방법Chemiluminescence

형태Liquid

용도(애플리케이션)Western Blot

Membrane CompatibilityNitrocellulose, PVDF

제품라인SuperSignal™

수량20 mL + 20 mL trial pack

SubstrateHorseradish Peroxidase

민감도Picogram to femtogram, low-femtogram to high-attogram

충분4 Mini-Gel Blots, 400 sq. cm of Membrane

배송 조건Approved for shipment at Room Temperature or on Wet or Dry Ice

Unit SizeEach