
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 405
Alexa Fluor 405로 결합된 염소 유래 anti-Rabbit IgG(H+L) 항체로, IHC, ICC/IF, Flow Cytometry에 적합합니다. 고순도 정제 및 교차 흡착 처리로 비특이적 결합을 최소화하며, 401/422 nm에서 발광합니다. 연구용으로 높은 신호 대 잡음비를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 405 |
| Excitation / Emission Max | 401 / 422 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_221605 |
| Target | IgG |
| Cross Adsorption | Against human IgG, human serum, mouse IgG, and bovine serum |
| Antibody Form | Whole Antibody |
Product Specific Information
- Product will be shipped at room temperature.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of target antibodies.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Alexa Fluor 350, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
- AP, APC, Biotin, Biotin-XX, Cyanine3, Cyanine5
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
- FITC, HRP, Pacific Blue, Pacific Orange, PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647, Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X, TRITC
- Custom conjugation available upon request.
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