Thermo Fisher Scientific BTK Monoclonal Antibody (OTI12H4), TrueMAB
BTK 단백질을 표적으로 하는 Mouse IgG3 단일클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 인간 시료 반응성, 무보존제, 동결건조 형태. 연구용으로 BTK 신호전달 및 면역 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI12H4 |
| Immunogen | Full-length human recombinant BTK protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL of distilled water to reach a final concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily. Mutations in BTK are associated with developmental blocks in human B-cell ontogeny and immunodeficiency disorders. BTK interacts with toll-like receptors (TLR4, 6, 8, 9) and key signal transduction proteins such as MyD88, TIRAP, and IRAK, but not TRAF-6, implicating BTK in lipopolysaccharide (LPS) signal transduction and immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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