Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), PerCP-Cyanine5.5, eBioscience
마우스 CD45.1 항원을 인식하는 A20 클론 단일클론 항체로, PerCP-Cyanine5.5 형광체가 결합되어 있습니다. Flow cytometry 분석에 최적화되어 있으며, 0.25 µg/test로 사용 가능합니다. 4°C에서 암소 보관하며, 연구용으로만 사용됩니다.
- 카탈로그번호
- 45-0453-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), PerCP-Cyanine5.5, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
View 62 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A20 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_925750 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- NovaFluor Blue 510, 610-30S, 660-40S
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- Super Bright 436, 600, 645, 702
- Request custom conjugation
Product Specific Information
The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. These strains include SJL/J, DA, STS/A, and RIII, and CD45.1 is expressed by all leukocytes in these strains.
Applications Reported:
This A20 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of SJL mouse splenocytes. Recommended usage ≤ 0.25 µg per test (100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Excitation: 488 nm
Emission: 695 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases and is essential for lymphocyte development and antigen signaling. Multiple isoforms arise through alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions. Isoform expression varies by cell type and physiological state. CD45 also promotes cell survival via integrin-mediated signaling and participates in DNA fragmentation during apoptosis. CD45RA is an isoform with restricted expression among lymphoid cell subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.