Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), PerCP-Cyanine5.5, eBioscience
마우스 CD45.2 항원을 인식하는 단클론 항체로, PerCP-Cyanine5.5로 결합되어 유세포분석(Flow Cytometry)에 적합합니다. C57BL/6, BALB/c 등 CD45.2 발현 마우스 균주에 사용 가능하며, 1 µg/test로 최적화되었습니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 45-0454-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), PerCP-Cyanine5.5, eBioscience
Applications
Tested Application
- Flow Cytometry (Flow)
Tested Dilution
- 1 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation/Emission Max | 489/679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_953592 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395~805
- Brilliant Violet 421~786
- eFluor 450, 506
- FITC
- NovaFluor Blue 510~660-120S
- PE, PE-Cyanine7, PE-eFluor 610
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
Description:
The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of BALB/c splenocytes. Recommended use ≤ 1 µg per test (in 100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Titrate antibody for optimal performance.
Fluorophore Properties:
- Excitation: 488 nm
- Emission: 695 nm
- Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, constituting about 10% of lymphocyte surface proteins. It regulates Src-family kinases, influencing lymphocyte development and antigen signaling. Multiple isoforms arise via alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45.2 also modulates integrin-mediated signaling, contributes to cell survival, and participates in DNA fragmentation during apoptosis. CD45RA is an isoform with restricted expression among lymphoid subtypes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.
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