Thermo Fisher Scientific Thrombomodulin Monoclonal Antibody (461714)
Thrombomodulin 단백질을 인식하는 Rat monoclonal antibody로, WB, IHC(F), ICC/IF, Flow Cytometry에 사용 가능. 마우스 시료 반응성. Protein A/G 정제, Lyophilized 형태로 제공되며 PBS+trehalose buffer에 보관. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 8–25 µg/mL |
| Immunocytochemistry (ICC/IF) | 8–25 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per million cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 461714 |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Thrombomodulin/BDCA-3 (Leu17–Ser517) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609392 |
Product Specific Information
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Thrombomodulin is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Thrombomodulin (TM), also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112, is a membrane glycoprotein expressed on endothelial cells. TM contains six EGF-like domains and a lectin-like N-terminal domain. It plays a critical role in anticoagulation by accelerating the activation of protein C, contributing to thrombo-resistance and reducing clot formation on endothelial surfaces.
Immunohistochemical staining of formalin-fixed tissue for TM has been used in studies of vascular tumors, meningiomas, and choriocarcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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