Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 650 / 786
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-70S / 690
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 590 / 660 / 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Request custom conjugation available

Product Specific Information

Description:
The W6/32 monoclonal antibody reacts with human MHC class I antigens (HLA-A, B, C), which are expressed on all nucleated cells and associated with β2-microglobulin. It recognizes a non-polymorphic epitope shared among HLA-A, B, and C products and immunoprecipitates both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and tested for flow cytometry using normal human peripheral blood cells
• Recommended use: 5 µL (0.6 µg) per test (in 100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample prior to combining antibodies with cells.
  • For single-color controls: use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for controlled fluorescence signatures and minimal spectral spillover.

Fluorescence Properties:

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

HLA-class I MHC antigens are intrinsic membrane glycoproteins expressed on nucleated cells and associated with β2-microglobulin. They are essential for immune recognition by cytotoxic T cells, playing a key role in antiviral and antitumor responses.
HLA and MHC antibodies are widely used in Immunopeptidomics, aiding in neoantigen identification and characterization via LC–MS/MS.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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