Thermo Fisher Scientific WTAP Polyclonal Antibody
WTAP 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. IHC(P)에서 1:50–1:200 희석 비율로 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. WTAP의 세포 내 역할 연구 및 단백질 발현 분석에 적합합니다.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 320–374 of Human WTAP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C (short term); for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2803185 |
Product Specific Information
- Purity is >95% by SDS-PAGE.
Target Information
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects approximately 1 in 10,000 infants and occurs in both sporadic and inherited forms.
The Wilms' tumor protein (WT1) binds to the DNA sequence GCGGGGGCG, a recognition site common to the Egr family of Zn²⁺ finger transcriptional activators, functioning as a transcriptional repressor.
WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may regulate mRNA splicing. WTAP localizes to nuclear speckles and regulates the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and impairs WT1 DNA-binding ability. Two isoforms exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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