Thermo Fisher Scientific ROCK1 Polyclonal Antibody
ROCK1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 Immunoprecipitation에 사용 가능. Antigen affinity chromatography로 정제되었으며, 4°C에서 보관. 세포 분화 및 이동 관련 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 400 and 450 of Human Rho-associated, coiled-coil containing protein kinase 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee
Target Information
ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 functions as a Caspase-3 substrate and contributes to myosin light chain (MLC) activation via direct phosphorylation and inhibition of MLC phosphatase. During apoptosis, ROCK1 is cleaved by caspase-3, removing its inhibitory domain and resulting in constitutive kinase activity. This activation leads to MLC phosphorylation and membrane blebbing, key events in the execution phase of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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