Thermo Fisher Scientific Fibronectin Monoclonal Antibody (3F12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:200

View 1 publication 1 publication

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Radioimmune Assays (RIA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Insect

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3F12

Immunogen

Human plasma fibonectin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985252

Product Specific Information

MA5-14737 has been successfully used in WB, ELISA, immunofluorescence, immunoprecipitation, and radioimmune assay applications, and reacts with human samples.

MA5-14737 detects Fibronectin which has a predicted molecular weight of approximately 259 kDa.

Product MA514737 is a smaller package size of MIF2601 (formerly sold as a Seradyn product).

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.