Thermo Fisher Scientific Histone H2A.X Recombinant Rabbit Monoclonal Antibody (JM06-42)
Recombinant Rabbit Monoclonal Antibody (JM06-42) for Histone H2A.X detection. Western blot, IHC, ICC 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Protein A 정제, 1 mg/mL 농도. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:4,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
This product is predicted to work in Immunoprecipitation at a dilution of 1:10–1:50.
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM06-42 |
| Immunogen | Synthetic peptide within N-terminal human Histone H2AX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2898994 |
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. DNA double-strand breaks (DSBs) in eukaryotes trigger phosphorylation of serine 139 at the C-terminus of H2AX, producing gamma-H2AX. This phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency.
Phosphorylation of H2AX is mediated by phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR. H2A.X contains 143 amino acids and is synthesized during both G1 and S-phase. Its mRNA exists in two forms: a short replication-type (~575 bases, no polyA) and a long basal-type (~1600 bases, with polyA). The gene maps to chromosome 11q23.2–q23.3 in humans.
H2A.X plays a critical role in chromatin structure and DNA repair by regulating the interaction of MDC1 (mediator of DNA damage checkpoint protein 1) with DNA damage sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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