Thermo Fisher Scientific GFAP Monoclonal Antibody (UMAB129), UltraMAB
인간 GFAP 단백질을 인식하는 UMAB129 클론의 마우스 단일클론 항체로, WB, IHC, ICC 등 다양한 실험에 사용 가능. 고순도 친화 크로마토그래피 정제, PBS/BSA/glycerol 완충액에 보관. 신경교세포 및 뇌종양 연구용 마커로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB129 |
| Immunogen | Full-length human recombinant GFAP protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.0 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. Neural stem cells also strongly express GFAP.
Antibodies to GFAP serve as useful markers for astrocytic cells and are widely used in brain tumor studies, as many astrocyte-derived tumors express GFAP. Additionally, GFAP is found in lens epithelium, Kupffer cells in the liver, and some salivary tumor cells, and has been reported in erythrocytes. It is used to distinguish astrocytes from other glial cells during development. Mutations in the GFAP gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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