Thermo Fisher Scientific PFKP Monoclonal Antibody (OTI3H8), TrueMAB
Human PFKP 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, Flow cytometry에 사용 가능. Lyophilized 형태로 제공되며, 높은 순도의 Affinity chromatography 정제 제품. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H8 |
| Immunogen | Full length human recombinant protein of human PFKP produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein kinases transfer phosphate groups from donors (typically ATP) to substrate proteins, mediating signal transduction in eukaryotic cells. They regulate metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
The protein kinase family includes over 500 gene products and is divided into 8 major groups based on catalytic domain sequences.
The AGC kinase group contains 63 kinases, including:
- PKA & PKG (cyclic nucleotide-regulated kinases)
- PKC (diacylglycerol/phospholipid-dependent kinases)
- RAC/Akt family
- ARK (G protein-coupled receptor kinases)
- RSK (ribosomal protein S6 kinases)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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