Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eFluor 450, eBioscience

Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eFluor 450

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f (integrin alpha 6), also known as VLA-6 alpha chain or platelet gpIc. CD49f is a 120 kDa transmembrane protein that associates with CD29 (integrin beta 1) to form VLA-6 and with CD104 (integrin beta 4) to form the α6β4 complex. It is expressed on T cells, monocytes, platelets, epithelial and endothelial cells, and germinal center B cells.
The antibody is cross-reactive with human, mouse, and bovine integrin alpha 6 and can block laminin binding.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Validated for flow cytometry on normal human peripheral blood cells. Recommended usage ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm (Violet laser compatible).

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD49f (Integrin alpha-6, ITGA6) is an integrin alpha chain that combines with beta chains to form various integrins such as α6β4 (TSP180) and α6β1 (VLA-6). Integrins mediate cell adhesion and signaling, influencing cellular shape, mobility, and proliferation.
They link extracellular matrix (ECM) components to intracellular cytoskeletal proteins and integrate with growth factor signaling.
CD49f is mainly expressed in epithelial cells and plays a structural role in hemidesmosomes. Over 18 alpha and 8 beta subunits with multiple splice variants are known in mammals.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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