Thermo Fisher Scientific Human AIF Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to the 14 amino acid N-terminus of human AIF.
PEP-0072 can be used as a blocking peptide with polyclonal antibody PA5-19947.

Target Information

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase-activated DNase, and several novel proteins.

Like the critical molecules cytochrome c and caspase-9 in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large-scale DNA fragmentation, which are the hallmarks of apoptosis, in both isolated nuclei and live cells under apoptotic stimuli.

AIF is highly conserved between human and mouse and is widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, resulting in severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF, and a related pseudogene has been identified on chromosome 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.