Thermo Fisher Scientific PolarScreen ER Beta Competitor Assay Kit, Red

Thermo Scientific™ PolarScreen™ ER Beta Competitor Assay Kit, Red

The PolarScreen™ ER Beta Competitor Assay Kit, Red provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential estrogen receptor (ER) beta ligands.
This kit uses insect cell-expressed, full-length, native (untagged) human ER beta (Cat. No. A15664) and a novel, high-affinity fluorescent estrogen ligand (Fluormone™ ES2) in a homogeneous, mix-and-read assay format.

This kit produces a red color read-out; a green version is also available (Cat. No. A15890). Both kits are suitable for determining IC50 values of competitive compounds.
Color selection depends on the spectral properties of the compounds to be screened and the available instrumentation.
For additional information or assistance, email drugdiscoverytech@lifetech.com.

Key Features

• Complete & ready-to-use out of the box—all reagents included, just add test compounds
• High throughput compatible—20 μL fluorescence polarization assays in a 384-well plate
• Convenient—available in bulk sizes for larger screening needs

How the Assay Works

Full-length ER beta is combined with a fluorescent estrogen ligand to form an ER–Fluormone™ ES2 complex.
This complex is then added to test compounds in multiwell plates.
Fluorescence polarization measurements determine relative affinity: competing test compounds displace Fluormone™ ES2 from ER beta, allowing rapid tumbling and resulting in low polarization values.
A shift in fluorescence polarization indicates the relative affinity of test compounds for ER beta.

All components are manufactured under strict quality parameters.
Product performance is guaranteed for 6 months after purchase per the Certificate of Analysis.
Contact us for bulk quantities or assistance with other nuclear receptor assay needs—from assay development to library screening.

Specifications