Thermo Fisher Scientific PolarScreen Progesterone Receptor Competitor Assay Kit, Red

Thermo Fisher Scientific PolarScreen™ Progesterone Receptor Competitor Assay Kit, Red

The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Red provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands.
This kit uses insect cell-expressed, human progesterone receptor ligand binding domain tagged with glutathione-S-transferase (GST) and a novel, high-affinity, fluorescent progesterone ligand (Fluormone™ PL Red) in a homogeneous, mix-and-read assay format.

This kit produces a red color read-out; a green version is also available. Both kits determine IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds and the available instrumentation.
For additional information or assistance, contact: drugdiscoverytech@lifetech.com

주요 특징

• Complete & ready-to-use — all reagents included, just add test compounds
• High throughput compatible — fluorescence polarization assays in 384-well plate format
• Convenient — components available in bulk sizes for large-scale screening

Assay Principle

An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently tagged progesterone ligand (Fluormone PL Red) in the presence of competitor test compounds in microwell plates.
Effective competitors prevent the formation of the PL Red/PR-LBD(GST) complex, resulting in a decrease in polarization value.
The shift in polarization value indicates the relative affinity of test compounds for PR-LBD(GST).

Contact us for bulk quantities or assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

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