Thermo Fisher Scientific Glucagon Polyclonal Antibody
Thermo Fisher Scientific Glucagon Polyclonal Antibody는 인간, 마우스, 랫트에서 반응하며 WB, IHC, ICC, ELISA에 사용 가능. Recombinant pro-glucagon(aa 21-89)을 면역원으로 한 rabbit IgG polyclonal 항체로, 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Glucagon Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| ELISA | 1 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 21–89 of human Pro-glucagon (NP_0020451) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.16 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805852 |
Product Specific Information
- Positive Samples: BxPC-3, 293T, Rat spleen
- Immunogen Sequence:
RSLQDTEEKS RSFSASQADP LSDPDQMNED KRHSQGTFTS DYSKYLDSRR AQDFVQWLMN TKRNRNNIA
Target Information
Glucagon is a 29-residue polypeptide hormone (MW 3482) produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), produced in the mucosa of the small and large intestine, extends the 29 amino acid sequence of pancreatic glucagon by 8 additional residues at the C-terminus.
The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogenesis, which counteract insulin action, leading to increased blood glucose levels.
Immunocytochemical studies have shown glucagon in pancreatic A (alpha) cells, comprising 15–20% of islet cells, located at the periphery of pancreatic islets.
Pathological manifestations of glucagon-type peptides are mainly associated with tumors or glucagonomas. Glucagon-specific antibodies are useful as cell and tumor markers in immunohistochemistry and as analytical tools in hormone quantification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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