Thermo Fisher Scientific IDH1 Polyclonal Antibody
IDH1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 인간 및 마우스 반응성, 고순도 정제 및 안정한 액상 형태 제공. 연구용으로만 사용.
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Thermo Fisher Scientific IDH1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 63–90 amino acids from the N-terminal region of human IDH1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion, Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2123157 |
Product Specific Information
This antibody is predicted to react with bovine and rat based on sequence homology.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one utilizing NAD(+) and the other NADP(+).
Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent enzymes localized to the mitochondrial matrix, and two NADP(+)-dependent enzymes, one mitochondrial and one cytosolic.
Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes, containing a PTS-1 peroxisomal targeting signal sequence.
This enzyme contributes to NADPH regeneration for intraperoxisomal reductions and reactions consuming 2-oxoglutarate, such as alpha-hydroxylation of phytanic acid, and plays a major role in cytoplasmic NADPH production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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