Thermo Fisher Scientific Phospho-IKB epsilon (Ser157) Polyclonal Antibody
Human IKB epsilon Ser157 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. 고순도 Protein A 정제 및 안정적 액상 형태로 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-IKB epsilon (Ser157) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human NFKBIE around the phosphorylation site of Ser157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NFKB1 or NFKB2 binds to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs (5′ GGGRNNYYCC 3′ or 5′ HGGARNYYCC 3′). Activation may require interaction with other transcription factors such as STAT6, AP1, and NFATC1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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