Thermo Fisher Scientific Human G-CSF Recombinant Protein

Applications

Western Blot (WB)

• Assay-dependent

Control (Ctrl)

• Assay-dependent

Product Specifications

Product Specific Information

This product contains purified recombinant human G-CSF protein with an N-terminal hexahistidine tag.
It may be used as a positive control in many immunological assays.

Target Information

G-CSF (Granulocyte Colony Stimulating Factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described.

This pleiotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells. G-CSF has specific effects on the proliferation, differentiation, and activation of hematopoietic cells. It is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukemia cells.

G-CSF is encoded by two distinct DNA sequences, resulting in a full-size, high-activity and a shorter, low-activity isoform. It is highly conserved among species and exerts its biological functions through interaction with its receptor expressed on hematopoietic progenitors, neutrophilic granulocytes, and certain carcinoma cell lines.

Clinically, G-CSF is used in several therapeutic applications including:

• Treatment of neonatal infections
• Therapy of acute myocardial infarction
• Granulocyte transfusion in neutropenic patients
• Management of severe infections and sepsis
• Therapy in chronic autoimmune neutropenia
• Treatment of acute myeloid leukemias, Sweet’s syndrome, and AIDS

G-CSF also serves as a marker protein for various carcinomas such as bladder cancer, and dysfunction of the protein has been linked to Kostmann Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.