Thermo Fisher Scientific PKC lambda/iota Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Immunogen sequence:
STMSHTVAGGG SGDHSHQVRV KAYYRGDIMI THFEPSISFE GLCNEVRDMC SFDNEQLFTM KWIDEEGDPC TVSSQLEL

Highest antigen sequence identity to the following orthologs:

• Mouse: 95%
• Rat: 95%

Target Information

The PKC family of serine/threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.

PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. The PKC family comprises at least eight members, which are differentially expressed and involved in a wide variety of cellular processes.

This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbol esters or diacylglycerol. It can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where it phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway.

This kinase is necessary for BCL-ABL-mediated resistance to drug-induced apoptosis, protecting leukemia cells against drug-induced apoptosis. A single exon pseudogene is mapped on chromosome X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.