Thermo Fisher Scientific B7-H4 (Immuno-Inhibitory Protein) Recombinant Rabbit Monoclonal Antibody (B7H4, 2652R)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Tested in direct ELISA (use antibody at 2–4 µg/mL for coating).
• For Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

B7-H4 (also known as B7x, VTCN1) is a costimulatory protein functioning as a negative regulator of T-cell mediated immunity.
Although its receptor is unknown, B7-H4 is believed to deliver inhibitory signals to T-cells, preventing proliferation, cell cycle progression, and interleukin-2 production.

• B7-H4-deficient mice show minimal immune alteration, suggesting its role in fine-tuning immune responses.
• Expressed on activated T-cells, B-cells, monocytes, and dendritic cells.
• Aberrant expression is associated with human cancers such as lung, breast, and ovarian cancer.
• Overexpression may help tumor cells evade immune surveillance by suppressing T-cell activity.
• VTCN1 may serve as a biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.