Thermo Fisher Scientific IgA Monoclonal Antibody (11-44-2), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/test

View 14 publications 14 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Bacteria, Human, Mouse, Rat

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

11-44-2

Conjugate

PE PE PE

View additional formats

• Biotin
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466115

Product Specific Information

Description: The 11-44-2 monoclonal antibody reacts with the alpha heavy chain of mouse IgA. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgM. IgA is expressed as a membrane-bound form as well as a soluble dimer. Fluorochrome conjugated 11-44-2 is used as a detection secondary for mouse IgA.

Applications Reported: 11-44-2 has been reported for use in flow cytometric analysis to detect the surface IgA on mouse B cells.

Applications Tested: This 11-44-2 antibody has been tested by flow cytometric analysis to detect a mouse IgA primary antibody and can be used at less than or equal to 0.2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgA is an immunoglobulin A antibody that is produced by B cells in response to an antigen. It is a crucial component of the mucosal immune system, which protects the body from pathogens that enter through mucosal surfaces such as the respiratory, gastrointestinal, and urogenital tracts. Mouse IgA is composed of two heavy chains and two light chains, and it is the most abundant immunoglobulin in mucosal secretions. It plays a vital role in neutralizing pathogens and preventing their attachment to mucosal surfaces. Mouse IgA is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.