Thermo Fisher Scientific L1CAM Monoclonal Antibody (OTI2H6), TrueMAB
L1CAM 단백질을 인식하는 Mouse Monoclonal Antibody로, Western Blot, IHC, ICC, Flow Cytometry 등에 사용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공되며, 인간 시료에 반응. 신경 발달 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H6 |
| Immunogen | Full length human recombinant protein of human L1CAM produced in HEK293T cell (Target Family: CD171 (L1CAM), UniProt ID: P32004-1, Antigen Range: 1–1257) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
L1CAM (CD171) is an axonal glycoprotein belonging to the immunoglobulin superfamily. Its ectodomain includes several immunoglobulin-like and fibronectin type III domains, connected to a conserved cytoplasmic region by a single transmembrane sequence.
This molecule plays a critical role in nervous system development, including neuronal migration and differentiation.
Mutations in the L1CAM gene are associated with X-linked neurological syndromes collectively known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus).
Alternative splicing of a neuron-specific exon may have functional significance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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