Thermo Fisher Scientific UNG Monoclonal Antibody (OTI1F8), TrueMAB
Thermo Fisher Scientific의 UNG 단클론 항체(OTI1F8)는 인간 UNG 단백질을 인식하는 Mouse IgG2a 형 항체로, WB, IHC(P), Flow Cytometry에 적합합니다. 고순도 친화 크로마토그래피 정제 제품이며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F8 |
| Immunogen | Full length human recombinant protein of human UNG produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These isoforms are generated by alternative splicing and the use of two differentially regulated promoters (PA and PB). The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively), and therefore, their mRNAs are not resolved as two species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence, which is not necessary for catalytic activity. The main role of UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. Expression levels of UNG1 and UNG2 differ between cell and tissue types and are regulated by the cell cycle, being generally higher in proliferating cells. UNG1 is a 304 amino acid protein, and UNG2 is a 313 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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