Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
TRITC 형광 표지된 Donkey anti-Rabbit IgG (H+L) 교차흡착 2차항체로, ICC/IF 실험에 적합. 높은 특이성과 낮은 비특이적 결합을 제공하며, 552/578 nm 파장에서 효율적인 형광 검출 가능. 연구용으로만 사용.
- 카탈로그번호
- A16034
- 판매단위
- pk
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Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles; store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534708 |
| Target | IgG |
| Cross Adsorption | Against mouse IgG and serum proteins |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP
- Biotin
- DyLight 350, 488, 550, 594, 650, 680, 755, 800
- FITC
- HRP
- Rhodamine
- Custom conjugation available upon request
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Product has been overfilled to ensure complete recovery. Centrifuge to remove particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG, or serum proteins.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit targets. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification. Typically produced by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (immunoaffinity chromatography, fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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