Thermo Fisher Scientific STIM1 Monoclonal Antibody (5A2)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

5A2

Immunogen

STIM1 (AAH21300, 24 a.a. approximately 685 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STIM1,uniProtId:Q13586-1,ncbiNodeId:9606,antigenRange:1-685,antigenLength:685,antigenImageFileName:H00006786-M01J_STIM1_Q13586-1_House_mouse.svg,antigenImageFileNamePDP:H00006786-M01J_STIM1_Q13586-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: SHSHSEKATG TSSGANSEES TAAEFCRIDK PLCHSEDEKL SFEAVRNIHK LMDDDANGDV DVEESDEFLR EDLNYHDPTV KHSTFHGEDK LISVEDLWKA WKSSEVYNWT VDEVVQWLIT YVELPQYEET FRKLQLSGHA MPRLAVTNTT MTGAVLKMTD RSHRQKLQLK ALDTVLFGPP LLTRHNHLKD FMLVVSIVIG VGGCWFAYIQ NRYSKEHMKK MMKDLEGLHR AEQSLHDLQE RLHKAQEEHR TVEVEKVHLE KKLRDEINLA KQEAQRLKEL REGTENERSR QKYAEEELEQ VREALRKAEK ELESHSSWYA PEALQKWLQL THEVEVQYYN IKKQNAEKQL LVAKEGAEKI KKKRNTLFGT FHVAHSSSLD DVDHKILTAK QALSEVTAAL RERLHRWQQI EILCGFQIVN NPGIHSLVAA LNIDPSWMGS TRPNPAHFIM TDDVDDMDEE IVSPLSMQSP SLQSSVRQRL TEPQHGLGSQ RDLTHSDSES SLHMSDRQRV APKPPQMSRA ADEALNAMTS NGSHRLIEGV HPGSLVEKLP DSPALAKKAL LALNHGLDKA HSLMELSPSA PPGGSPHLDS SRSHSPSSPD PDTPSPVGDS RALQASRNTR IPHLAGKKAV AEEDNGSIGE ETDSSPGRKK FPLKIFKKPL KK

Target Information

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.