Thermo Fisher Scientific CDKN2A/p14ARF Polyclonal Antibody, FITC
CDKN2A/p14ARF 단백질 검출용 FITC 결합 폴리클로날 항체. Western blot, ELISA, IP 등 다양한 응용에 적합. 인간, 생쥐, 랫드 반응성. 항원 특이적 친화 크로마토그래피 정제. 암 억제 단백질 연구 및 세포주기 조절 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to C-terminal amino acids 94–111 of Human p14ARF (Sequence: QRLGQQPLPRRPGHDDGQ) |
| Conjugate | FITC (Fluorescein) |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These proteins interact with upstream tumor suppressors such as retinoblastoma protein and p53, playing key roles in cell senescence, cell cycle control, and tumor suppression. p14ARF (or P19ARF in mouse) can induce both G1 and G2 phase arrest in a p53-dependent manner. Differences in ARF function between species may contribute to varying tumor susceptibilities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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