Thermo Fisher Scientific Phospho-EphB2 (Tyr317) Polyclonal Antibody
Phospho-EphB2 (Tyr317) 폴리클로날 항체는 Rabbit IgG 기반으로 Western blot에 최적화되어 있습니다. Chicken, Mouse, Rat에 반응하며 Tyr317 인산화 EphB2 단백질을 특이적으로 인식합니다. Affinity chromatography로 정제되었으며, -20°C에서 보관합니다.
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Applications and Tested Dilution
- Western Blot (WB): 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 of chicken EPHB2 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2095687 |
Product Specific Information
This antibody is predicted to react with bovine, human, mouse, Xenopus, and zebrafish based on 100% sequence homology.
It is specific for the ~46 kDa EphrinB protein phosphorylated at Tyr317. Immunolabeling can be blocked by lambda-phosphatase treatment.
Target Information
EPHB2 is a receptor tyrosine kinase belonging to the ephrin receptor family. Members of this family play critical roles in biological processes such as nervous system development, angiogenesis, and neural synapse formation and maturation.
Ephrins are divided into two classes:
- Ephrin-A (EFNA): Anchored to the membrane via a glycosylphosphatidylinositol linkage
- Ephrin-B (EFNB): Transmembrane proteins
Eph receptors are similarly divided into two groups based on extracellular domain sequence similarity and ligand-binding affinity. Ephrin receptors represent the largest subgroup within the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and is essential for vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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