Thermo Fisher Scientific HMGN1 Polyclonal Antibody
HMGN1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체로, 4°C 보관. 인간 및 마우스 반응성.
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Thermo Fisher Scientific HMGN1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 5–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 90 and 100 of human high-mobility group nucleosome binding domain 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription.
HMG-14 and HMG-17 also function as architectural elements, altering chromatin fiber structure and facilitating transcription.
They modify the nucleosomal organization of the 30 nm chromatin fiber and mediate unfolding of higher-order chromatin, improving DNA accessibility.
Clustering of HMG proteins and linker histones into distinct domains may lead to structural and functional heterogeneity along chromatin fibers.
HMG-14 and HMG-17 are also components of mouse oocyte and embryonic chromatin, linking chromatin structure to embryonic development progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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