Thermo Fisher Scientific HMGN1 Polyclonal Antibody
HMGN1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제된 액상 제품이며, 4°C에서 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific HMGN1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 5–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 90 and 100 of human high-mobility group nucleosome binding domain 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
High-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. These proteins act as architectural elements, altering chromatin structure and facilitating transcription from chromatin templates. HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber and promote unfolding of higher-order chromatin structures, thereby improving DNA accessibility. They are also constitutive components of mouse oocyte and embryonic chromatin, linking chromatin structure to embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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