Thermo Fisher Scientific CD1d Monoclonal Antibody (51.1), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
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Product Specifications

Available Formats

• Unconjugated
• APC
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family similar to non-polymorphic MHC Class I-like molecules. CD1d is a conserved transmembrane receptor of the Immunoglobulin Superfamily and can associate with beta-microglobulin or exist independently, suggesting different mechanisms for glycolipid antigen presentation to CD1d-reactive NKT cells.

CD1d expression is found on peripheral B cells, resting monocytes, cortical thymocytes, and intestinal epithelial cells (IEC) with polarized expression. It is also found at low levels in hepatocytes and is highly expressed in HCV-infected livers compared to normal controls.

The 51.1 monoclonal antibody has functional activity, blocking interactions between CD1d-transfected cells and NKT cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on normal human peripheral blood cells
  • Recommended use: 5 µL (0.125 µg) per test (final volume 100 µL; cell count 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin-independent expression has been shown on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of MHC-like glycoproteins. The CD1d gene encodes a transmembrane glycoprotein forming heterodimers with beta-2-microglobulin and mediating lipid/glycolipid antigen presentation to T cells.

The human genome contains five CD1 family genes clustered on chromosome 1. These members differ in localization and lipid ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail.

Associated Diseases: Mycobacterium Malmoense, Autoimmune Disease.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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