Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), PE, eBioscience
마우스 CD1d 단백질을 검출하기 위한 PE 결합 단클론 항체로, Flow Cytometry 분석에 최적화되어 있습니다. Rat IgG2b, kappa 아이소타입이며 0.125 µg/test로 사용 가능합니다. 565/576 nm의 형광 특성을 가지며 4°C 암소 보관을 권장합니다.
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Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
Publications: View 25 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465482 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. CD1d associates with beta2m and is functionally similar to MHC class II.
1B1 detects CD1d at varying levels on mouse leukocytes and beta2m-associated CD1d.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.125 µg/test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. It can also be expressed independently on human intestinal epithelial cells.
CD1d belongs to the CD1 family of MHC-like glycoproteins and mediates presentation of lipid and glycolipid antigens to T cells.
The human genome contains five CD1 family genes on chromosome 1, differing in localization and lipid ligand specificity.
CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail.
Associated diseases include Mycobacterium Malmoense infection and autoimmune disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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