Thermo Fisher Scientific ARK5 Polyclonal Antibody
Thermo Fisher Scientific의 ARK5 Polyclonal Antibody는 인간 NUAK1(ARK5) 단백질을 인식하는 염소 유래 IgG 항체입니다. ICC/IF 및 유세포분석(Flow Cytometry)에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 암모늄 황산 침전으로 정제되었으며, -20°C에서 보관합니다.
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Thermo Fisher Scientific ARK5 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (QNRPRPQYLKRYRNR) corresponding to internal amino acids of NUAK1 (aa 617–632) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_11004521 |
Product Specific Information
- Predicted to react with rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
NUAK1 (ARK5) is a serine/threonine kinase involved in various cellular functions. Overexpression of this kinase has been associated with tumor invasion and metastasis, particularly in colorectal neoplasms.
It regulates cellular senescence and ploidy by phosphorylating Ser-464 of LATS1, influencing its stability.
Controls cell adhesion through regulation of the myosin protein phosphatase 1 (PP1) complex.
Participates in DNA damage response by phosphorylating p53/TP53 at Ser-15 and Ser-392 and contributing to transcription activation at the CDKN1A/WAF1 promoter.
Acts as a tumor malignancy-associated factor under AKT1 regulation, suppresses Fas-induced apoptosis via CASP6 phosphorylation, and regulates UV-induced DNA damage response through CDKN1A phosphorylation and degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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