Thermo Fisher Scientific SPI-B Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.2–1 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
WGQQKGNRKR MTYQKLARAL RNYAKTGEIR KVKRKLTYQF DSALLPAVRR

• Short-term storage: 2–8°C (up to 1 week)
• Long-term storage: -20°C (store in small aliquots to prevent freeze-thaw cycles)

Predicted homology:
Cow: 100%
Dog: 100%
Guinea Pig: 100%
Horse: 100%
Human: 100%
Mouse: 100%
Rabbit: 100%
Rat: 100%

Target Information

Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding. Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1/PU.1 related, JUN, and TBP.

As a sequence-specific transcriptional activator, it binds to the PU-box (a purine-rich DNA sequence, 5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Two isoforms of Spi-B are reported.

Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon-producing cells, and promotes their development and blocks viral replication.

Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells, where it helps in B-cell receptor (BCR) signaling, normal B-cell development, and antigenic stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.