Thermo Fisher Scientific SDHA Monoclonal Antibody (2E3GC12FB2AE2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

View 81 publications 81 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

1 µg/mL

-

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2E3GC12FB2AE2

Immunogen

Full length native protein (purified) corresponding to Cow SDHA. Purified mitochondrial complex II (Cow). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SDHA,uniProtId:P31039-1,ncbiNodeId:9913,antigenRange:1-665,antigenLength:665,antigenImageFileName:459200_SDHA_P31039-1_House_mouse.svg,antigenImageFileNamePDP:459200_SDHA_P31039-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline, pH 7.5

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10838019

Product Specific Information

ICC application requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).

Positive control: Human heart mitochondria.

Target Information

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.