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Thermo Fisher Scientific SDHA Monoclonal Antibody (2E3GC12FB2AE2)
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Thermo Fisher Scientific SDHA Monoclonal Antibody (2E3GC12FB2AE2)

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SDHA 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단일클론 항체로, WB, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. 인간, 마우스, 랫, 소 등에서 반응하며, 고순도 IgG1 항체. 연구용으로 미토콘드리아 복합체 II 분석에 적합.

판매단위
pk
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Thermo Fisher Scientific 459200 SDHA Monoclonal Antibody (2E3GC12FB2AE2) 100 ug pk판매 단위 pk
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Thermo Fisher Scientific · Thermo Fisher Scientific SDHA Monoclonal Antibody (2E3GC12FB2AE2)

Thermo Fisher Scientific SDHA Monoclonal Antibody (2E3GC12FB2AE2)

Applications and Tested Dilutions

Application Tested Dilution Publications
Western Blot (WB) 0.1 µg/mL 81
Immunohistochemistry (IHC) - 4
Immunohistochemistry (Paraffin) (IHC (P)) - 2
Immunohistochemistry (Frozen) (IHC (F)) Assay-dependent -
Immunocytochemistry (ICC/IF) 1:200 4
Flow Cytometry (Flow) 1 µg/mL -
ELISA - 1
Immunoprecipitation (IP) - 1
Miscellaneous PubMed (Misc) - 1

Product Specifications

Property Description
Species Reactivity Bovine, Human, Mouse, Rat
Published Species Dog, Human, Mouse, Rat
Host/Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Clone 2E3GC12FB2AE2
Immunogen Full length native protein (purified) corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).
Conjugate Unconjugated
Form Liquid
Concentration 1 mg/mL
Purification IgG fraction
Storage Buffer HEPES buffered saline, pH 7.5
Contains 0.02% sodium azide
Storage Conditions 4°C
Shipping Conditions Wet ice
RRID AB_10838019

Product Specific Information

  • ICC application requires heat-induced antigen retrieval when aldehydes are used as fixatives.
    Use 20 min incubation at 90–100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).
  • Positive control: Human heart mitochondria.

Target Information

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain.
The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane.
Mutations in this gene have been associated with mitochondrial respiratory chain deficiency (Leigh Syndrome).
A pseudogene has been identified on chromosome 3q29.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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