
Thermo Fisher Scientific SDHA Monoclonal Antibody (2E3GC12FB2AE2)
SDHA 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단일클론 항체로, WB, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. 인간, 마우스, 랫, 소 등에서 반응하며, 고순도 IgG1 항체. 연구용으로 미토콘드리아 복합체 II 분석에 적합.
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Thermo Fisher Scientific SDHA Monoclonal Antibody (2E3GC12FB2AE2)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | 81 |
| Immunohistochemistry (IHC) | - | 4 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:200 | 4 |
| Flow Cytometry (Flow) | 1 µg/mL | - |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Dog, Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E3GC12FB2AE2 |
| Immunogen | Full length native protein (purified) corresponding to Cow SDHA. Purified mitochondrial complex II (Cow). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | HEPES buffered saline, pH 7.5 |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10838019 |
Product Specific Information
- ICC application requires heat-induced antigen retrieval when aldehydes are used as fixatives.
Use 20 min incubation at 90–100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol). - Positive control: Human heart mitochondria.
Target Information
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain.
The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane.
Mutations in this gene have been associated with mitochondrial respiratory chain deficiency (Leigh Syndrome).
A pseudogene has been identified on chromosome 3q29.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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