Thermo Fisher Scientific SDHB Monoclonal Antibody (21A11AE7)
SDHB 단백질을 검출하기 위한 Thermo Fisher의 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. Bovine, Human, Mouse, Rat에 반응하며, 4°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | View 15 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 1 µg per 10^6 cells | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 21A11AE7 |
| Immunogen | Bovine Heart Complex II, with traces of Complex III |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2532233 |
Product Specific Information
- Positive control: Human heart mitochondria.
Target Information
Complex II of the respiratory chain is specifically involved in the oxidation of succinate, carrying electrons from FADH to CoQ.
The complex comprises four nuclear-encoded subunits localized in the mitochondrial inner membrane.
The iron-sulfur subunit is highly conserved, containing three cysteine-rich clusters that may form the enzyme’s iron-sulfur centers.
Mutations in this gene are associated with paragangliomas and pheochromocytoma, linking mitochondrial dysfunction to tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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