Thermo Fisher Scientific Cycloxygenase-2 (COX-2) Monoclonal Antibody (COX2/2377)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• 1–2 µg/mL

Product Specifications

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins.
The conversion of arachidonate to prostaglandin H2 is a two-step reaction:

1. A cyclooxygenase (COX) reaction converting arachidonate to prostaglandin G2 (PGG2).
2. A peroxidase reaction reducing PGG2 to prostaglandin H2 (PGH2).

COX2 is constitutively expressed in certain tissues such as endothelium, kidney, and brain, and is up-regulated under pathological conditions like cancer and inflammation.
It is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 is critical for prostaglandin E2 (PGE2) production, influencing motility, proliferation, and apoptosis resistance.
COX2 activity is naturally inhibited by calcitriol (active Vitamin D) and repressed by glucocorticoids.
COX2 is a known target of NSAIDs such as aspirin, which reduce pain and inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.