Thermo Fisher Scientific CENP-F/Mitosin Polyclonal Antibody
CENP-F/Mitosin 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 적합. 4°C 보관, 액상 형태로 제공. 세포 분열 과정에서 염색체 분리 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2350 and 2400 of human centromere protein F (Mitosin) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2, it associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests a role in chromosome segregation during mitosis. It may form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft-versus-host disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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