Thermo Fisher Scientific BID Monoclonal Antibody (UMAB139), UltraMAB
BID 단백질을 인식하는 UMAB139 클론의 마우스 단일클론 항체입니다. Western blot 및 IHC(P)에서 검증되었습니다. 고순도의 액상 포맷으로 제공되며, PBS/BSA/glycerol 완충액에 보관됩니다. 세포 사멸 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB139 |
| Immunogen | Full length human recombinant protein of human BID produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. It belongs to the BCL-2 family of cell death regulators and mediates mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal fragment translocates to mitochondria to trigger cytochrome c release. BID acts as a pro-apoptotic molecule linking caspase-8 activation to mitochondrial apoptosis pathways. In living cells, BID typically exists in an inactive cytosolic form and becomes cleaved and activated by caspase-8 in response to TNF-alpha or Fas ligand stimulation. The cleaved 15 kDa fragment (p15) translocates to mitochondria and induces cytochrome c release, leading to apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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