Thermo Fisher Scientific JMJD1A/JHDM2A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

JMJD1A is a histone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in the histone code. It preferentially demethylates mono- and dimethylated H3 Lys-9 residues, with a preference for dimethylated residues, while showing weak or no activity on trimethylated H3 Lys-9.
Demethylation of Lys residues generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation by participating in recruitment to androgen-receptor target genes, resulting in H3 Lys-9 demethylation and transcriptional activation.
It is also involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1, which are required for packaging and condensation of sperm chromatin. Furthermore, JMJD1A contributes to obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.