Thermo Fisher Scientific Superoxide Dismutase 1 (SOD1) (Antioxidant Enzyme) Monoclonal Antibody (SOD1, 4248)
SOD1 단백질을 인식하는 mouse monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. Human 시료에 반응하며, Protein A/G로 정제된 액상 형태로 제공됩니다. 항산화 효소 연구 및 ALS 관련 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SOD1, 4248 |
| Immunogen | Recombinant fragment (around aa14–148) of human SOD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Formalin-fixed tissues require heating in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein that acts as a homodimer to convert harmful superoxide radicals into molecular oxygen and hydrogen peroxide. The other isozyme is mitochondrial. Mutations in this gene are associated with familial amyotrophic lateral sclerosis (ALS). Rare transcript variants have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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